rabbit polyclonal anti cacna1c  (Alomone Labs)

Journal: International Journal of Molecular Sciences

Article Title: Thyroid Hormone Upregulates Cav1.2 Channels in Cardiac Cells via the Downregulation of the Channels’ β4 Subunit

doi: 10.3390/ijms251910798

Figure Lengend Snippet: Relative mRNA expression and α1c protein abundance in H9c2 cells after incubation with T3. ( A , B ) Means ± SEMs of relative α1c subunit mRNA expression and protein abundance in H9c2 cells from three separate experiments. Insets show representative blots under control conditions (lanes a, c, e, and g) and 2, 6, 24, and 48 h after T3 treatment (lanes b, d, f, and h, respectively). Tubulin bands were used to normalize α1c subunit density values. In panels ( A , B ) each symbol represents a separate experiment. Original blots are presented in . * p < 0.05. ** p < 0.01.

Article Snippet: The antibody sources were polyclonal anti-rabbit α1c (ACC-003, 1:200; Alomone Labs, Jerusalem, Israel) and polyclonal anti-rabbit CACNB4 (1:500, 1:1000, A9304; ABclonal Technology, Woburn, MA, USA).

Techniques: Expressing, Incubation, Control

Journal: International Journal of Molecular Sciences

Article Title: Thyroid Hormone Upregulates Cav1.2 Channels in Cardiac Cells via the Downregulation of the Channels’ β4 Subunit

doi: 10.3390/ijms251910798

Figure Lengend Snippet: Relative α1c mRNA expression and protein abundance in H9c2 cells after Cavβ4 subunit overexpression. ( A ) Means ± SEMs of relative Cavβ4 protein abundance under control conditions and after Cavβ4 overexpression ( n = 3). The inset shows representative blots of the Cavβ4 subunit and GAPDH bands; the latter was used to normalize Cavβ4 subunit density values. Original blots are presented in . ( B ) Means ± SEMs of relative α1c subunit mRNA expression from control and Cavβ4 overexpression experiments ( n = 14). ( C ) Means ± SEMs of α1c protein abundance from control and Cavβ4 overexpression experiments ( n = 10). The inset shows representative blots of α1c subunit and tubulin bands under control conditions (lanes a and c) and after Cavβ4 overexpression (lanes b and d) from two separate experiments. Original blots are presented in . In panels ( A , B , C ), each symbol represents a separate experiment. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The antibody sources were polyclonal anti-rabbit α1c (ACC-003, 1:200; Alomone Labs, Jerusalem, Israel) and polyclonal anti-rabbit CACNB4 (1:500, 1:1000, A9304; ABclonal Technology, Woburn, MA, USA).

Techniques: Expressing, Over Expression, Control

Journal: International Journal of Molecular Sciences

Article Title: Thyroid Hormone Upregulates Cav1.2 Channels in Cardiac Cells via the Downregulation of the Channels’ β4 Subunit

doi: 10.3390/ijms251910798

Figure Lengend Snippet: Role of Cavβ4 in pCREB regulation by T3. Schematic showing signaling mechanisms hypothetically involved in thyroid hormone actions on the CACNA1C gene via pCREB and the release of Cavβ4 inhibition of α1c subunit expression in ventricular cardiac cells.

Article Snippet: The antibody sources were polyclonal anti-rabbit α1c (ACC-003, 1:200; Alomone Labs, Jerusalem, Israel) and polyclonal anti-rabbit CACNB4 (1:500, 1:1000, A9304; ABclonal Technology, Woburn, MA, USA).

Techniques: Inhibition, Expressing

Journal: iScience

Article Title: Exendin-4 promotes retinal ganglion cell survival and function by inhibiting calcium channels in experimental diabetes

doi: 10.1016/j.isci.2023.107680

Figure Lengend Snippet: Involvement of intracellular Ca 2+ , CaM, calcineurin and PP1 in suppression of I LCa by Ex-4 (A and B) Representative recordings from an RGC at −10 mV, showing that during internal infusion of Ca 2+ -free solution (containing 10 mM BAPTA; control), Ex-4 failed to suppress the I LCa , and summary data are shown in (B) (n = 6). (C and E) Current traces of two RGCs, showing that during internal dialysis of 5 mg/mL heparin (C), but not ryanodine (50 μM, E), the Ex-4-induced suppression of I LCa was seen. (D and F) Bar charts summarizing the effects of Ex-4 on the I LCa amplitudes in the presence of heparin (n = 7) (D) or ryanodine (n = 7) (F). (G, I, and K) Representative recordings obtained from three different RGCs showing that Ex-4 no longer reduced the I LCa amplitudes during the internal infusion of 100 μM W-7 (G), 50 μM FK-506 (I) and 1 μM OA (K) to block CaM, calcineurin and PP1, respectively. (H, J, and L) Bar charts summarizing the results regarding the effects of W-7 (n = 7) (H), FK-506 (n = 8) (J) or OA (n = 8) (L). (M and N) Current traces of an RGC, showing that perfusion of C2 Ceramide (500 nM) suppressed the I LCa (M), and summary data are shown in (N) (n = 6). Data are presented as mean ± SD, n.s., p > 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001 by paired t test. (O) Representative immunoblots showed changes in the expression of L-VGCC subunits Cav1.2 and p -Cav1.2 in membrane components in retinas from the control, DM rats treated with or without Ex-4 eye drops. Na + -K + -ATPase served as loading control. (P and Q) Densitometric analysis revealing a significant increase in Cav1.2 (P) and p -Cav1.2 (Q) protein levels in DM retinas. Topical administration of Ex-4 significantly decreased Cav1.2 and p -Cav1.2 levels in DM retinas. (R) Quantitative analysis of p -Cav1.2 relative to Cav1.2 total protein is shown in the bar graph. The level of p -Cav1.2/Cav1.2 did not change in DM retinas, but significantly decreased in the DM + Ex-4 group. n = 3 for each group. Data are presented as mean ± SD. n.s., p > 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001 by post hoc Tukey’s multiple comparisons test after one-way ANOVA. See also Figure S3 .

Article Snippet: Rabbit polyclonal anti-Cav1.2 (CACNA1C) , Alomone , Cat#ACC-003; RRID: AB_2039771.

Techniques: Blocking Assay, Western Blot, Expressing, Membrane, Eye Drops

Journal: iScience

Article Title: Exendin-4 promotes retinal ganglion cell survival and function by inhibiting calcium channels in experimental diabetes

doi: 10.1016/j.isci.2023.107680

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Cav1.2 (CACNA1C) , Alomone , Cat#ACC-003; RRID: AB_2039771.

Techniques: Recombinant, Protease Inhibitor, Software